GRK 2905 – Ultrafast Nanoscopy
From single particle dynamics to cooperative processes
GRK Colloquium:
Jan Vogelsang & Gerti Beliu
July 4 @ 14:00 – 16:00
2 pm
From single‐molecule fluorescence spectroscopy to (fast) super‐resolution microscopy
Super‐resolution (SR) microscopy has revolutionized far‐field fluorescence imaging by overcoming the diffraction limit of light. The technique relies fundamentally on the ability to detect single fluorescent molecules, and the first part will focus on achieving this, which requires carefully optimized technical and photophysical needs, including the manipulation of fluorophore behavior through photoinduced redox reactions. The second part will focus on a novel approach using DNA origami nanoantennas to dramatically enhance fluorescence
signals in single‐molecule experiments. Such nanoantennas can increase the fluorescence of single molecules by an order of magnitude, which increases the time resolution and facilitates the observation of ultrafast processes such as the
diffusive barrier crossing events between two potential energy minima in disordered proteins.
Dr. Jan Vogelsang
University of Regensburg
Institute of Experimental and Applied Physics
3 pm
Click. Tag. Image –A Hitchhiker’s Guide to Molecular Bioimaging
Super‐resolution microscopy holds the promise of visualizing molecular processes in cells with nanometer precision. However, realizing this potential requires labeling strategies that are not only bright and site‐specific, but also biologically orthogonal. Many commonly used labeling approaches interfere with protein function or fail to access conformationally restricted or sterically masked epitopes in live‐cell environments. In our work, we develop and apply biochemical labeling strategies that combine genetic code expansion, bioorthogonal click chemistry, and fluorogenic dyes to overcome these limitations. By minimizing linkage errors and enabling stoichiometric labeling under native conditions, we aim to push the limits of resolution, specificity, and functional integrity in molecular imaging. This talk will provide an overview of our toolbox, recent applications, and future directions for nanoscale imaging of proteins in living systems.
Venue: RUN auditorium
Prof. Dr. Gerti Beliu
University of Regensburg
Institute of Pharmacy